Lectins: Analytical Technologies

Hardback  456 pages  ISBN 9780444530776      £83.00

• Reviews up-to-date techniques, including practical hints for laboratory work
• Provides overview of lectin e-resources and several color illustrations
• Includes a 'look-up' table detailing lectin specificity

Lectins: Analytical Technologies covers both analytical and biological aspects of lectins (functional carbohydrate (complex sugar) recognition proteins) and provides researchers in the field with a resource containing background information and 'look-up' tables detailing lectin specificity and structures. Also included are methods and practical tips for designing new lectins from existing non-lectin proteins, automated approaches to lectin proteomics and high resolution mass spectrometry techniques.

This book will be of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields who are involved in the study of lectin structures or who utilize lectins as analytical tools. The study of lectins and their employment in analytical settings spans a range of fields including crystallography and lectin structure databases, carbohydrate microarrays for lectin characterization and glycotope identification and proteomic approaches to the functional as well as identification of bacterial adhesins. It also covers generation of lectins from enzymes and probing cell-surface lectins with neoglycoconjugates

Of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields.

Contents

• Lectins: Analytical Tools from Nature (C.L. Nilsson).
• X-ray Crystallography and Lectin Structure Databases (U. Krengel, A. Imberty).
• Applications of NMR Spectroscopy to the Study of Lectin-Sugar Interactions (P. Groves, A. Canales, M.I. Chavez, M. Palczewska, D. Diaz, F. J. Canada, J. Jimenez-Barbero).
• Microcalorimetry: A Tool to Study Lectin-Carbohydrate Interactions (T.K. Dam. C. Fred Brewer).
• Bioanalytical Studies based on Lectin-Carbohydrate Interactions as Measured by Ellipsometry and Surface Plasmon Resonance Techniques (J. Masarova, F. Winquist, B. Danielsson).
• Use of Natural and Synthetic Glycolipid Libraries in Defining Lectins from Pathogens (J. Natunen).
• Carbohydrate Microarrays for Lectin Characterization and Glycotope Identification (D. Wang, A.M. Wu).
• Glycoproteomics based on Lectin Affinity Chromatographic Selection of Glycoforms (F.E. Regnier, K. Jung, S.B. Hooser, C.R. Wilson).
• Miniaturized Lectin Affinity Chromatography (X. Mao, J. Qin, B. Lin).
• Frontal Affinity Chromatography: Systematization for Quantitative Interaction Analysis between Lectins and Glycans (S. Nakamura-Tsuruta, N. Uchiyama, J. Kominami, Jun Hirabayashi).
• Technical Aspects of Glycoprotein Enrichment (M. Novotny, M. Madera, Y. Mechref).
• Proteomic Approaches to the Functional Identification of Bacterial Adhesins (E. Carlsohn, C.L. Nilsson).
• The Use of Lectins in Bioaffinity MALDI Probes (C. Lebrilla).
• Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Lectin Characterization (J.T. Adamson, K. Hakansson).
• Carbohydrate-binding Endoglycosidases as Microbial Diagnostic Reagents:Chitinase as a Fungal Capture/Label Agent (R. Laine, J.W.-C. Lo, B.C.-R. Zhu).
• Generation of Lectins from Enzymes: Use of Inactive Endosialidase for Polysialic Acid Detection (A. Jokilammi, M. Korja, E. Jakobsson, J. Finne).
• Probing Cell Surface Lectins with Neoglycoconjugates (E.M. Rapoport, E.I. Kovalenko, I.M. Belyanchikov, N. Bovin).